ACEL, Inc. is CRO of Cell-based Assays.

List of Cell-based Assays

Cosmetology field(cell-based assays)

• Melanin production suppression test
     (suppression of melanin synthesis for whitening)
• Fibroblast function promotion test
    (collagen promotion, hyaluronic acid production, etc.)
• Epidermal cell test: ultraviolet (UV) protection of skin
    (protects skin from stress, such as UV rays)
• Skin moisturizing/barrier function gene expression analysis
     (moisturizing using epidermal cells and promoting barrier function)
• Dry stress protection test for skin and cornea
    (protection of dry skin and improvement of dry eye)
• An activation test of dermal papilla cells
    (the functional substance that promotes hair formation)
• An activation test of various cells: a cell proliferation promotion/suppression test
     (cosmetology, wound healing, suppression of cancer cells, etc.)
• Cell migration test
     (wound healing, etc.)
• Atopic dermatitis improvement test
     (search for substances that improve atopy, etc.)

Cosmetology field (biochemical test)

• Collagenase activity inhibition test
     (control of collagen degradation)）
• Tyrosinase activity inhibition test
     (inhibition of melanin synthesis for whitening)

Health field (immune system)

• Immune function regulation test
     (cytokine gene expression in macrophages)
• Histamine release suppression test
     (excessive allergic reaction suppression by histamine)
• Cytokine production measurement test
     (measurement of cytokines released from various cells)
• Multiplex measurement
     (simultaneous measurement of multiple biomolecules, including cytokines)
• Immune (blood) function improvement test
     (ability to promote differentiation into each blood cell)
• Enteritis suppression test
     (effect of suppressing intestinal inflammation)
• Pulmonary function improvement test
     (effect of suppressing intestinal inflammation)

Health field(anti-obesity system)

• Fat differentiation suppression test
     (search for functional substances expected to have a diet effect)
• Lipolysis promotion test
     (search for functional substances expected to have a diet effect)
• Fat function improvement test
     (search for functional substances expected to have a diet effect)
• Cell mitochondrial activation test
    (search for functional substances that promote fat burning, etc.)
• Sugar uptake test of various cells
     (search for substances with anti-diabetes and diet effects)

Health field(blood system)

• Blood vessel function improvement test
    (vasodilatory action, antithrombotic action, etc.)
• Angiogenesis test
     (search for wound healing agents and cancer suppressants)
• Antiarteriosclerosis test
    (search for drugs that suppress the occurrence and progression of arteriosclerosis)

Health field (bone/cartilage system)

• Bone metabolism test using osteoblasts and osteoclasts
     (supporting bone health)）
• Chondrocyte function improvement test
     (treatment and prevention of joint diseases, etc.)

Health field (liver system)

• Liver function improvement test
     (correlation between drug and CYP as well as drug efficacy)

Health field (nervous system)

• Nerve cell function promotion test
     (improvement effect on neurological diseases)
• NGF (Nerve growth factor) production promotion test
     (nerve cell differentiation and regeneration, neurodegenerative diseases)

Health field(muscular system)

• Muscle building test using myoblasts
    (increasing and maintaining muscle mass)
• Energy production promotion test using myotube cells
    (energy production promotion effect of muscle mass)

Health field(others)

• Nicotinamide adenine dinucleotide phosphate oxidase activity measurement
     (control of active oxygen production)
• Apoptosis induction test
     (induction of cell death by apoptosis, etc.)
• Cancer cell infiltration inhibition test
     (cancer cell expansion, metastasis suppression, etc.)
• Autophagy promotion test
     (cell autophagy activation, etc.)
• Energy metabolism test
     (test using a flux analyzer)

Induced pluripotent stem (iPS) cells

• Tests using
     iPS cells (examination of iPS cell culture conditions and differentiation induction conditions, etc.)

Animal experiment (alternative field)

• Melanin production suppression test (three-dimensional cultured skin model)
     (whitening agent, raw material for whitening cosmetics)

Molecular biology field

• Gene expression analysis
     (Ribonucleic acid [RNA] is extracted from cells and subjected to real-time polymerase chain reaction)
• Comprehensive gene expression analysis (Deoxyribonucleic acid [DNA] microarray)
     (measurement of each RNA amount in cells)
• Gene knockdown test using small interfering RNA (siRNA)
    (inhibition of gene expression in cells)
• Cell-forced gene expression test
     (overexpression)
• Western blotting
     (protein expression analysis using simple western blotting)

Base material evaluation

• Base material evaluation test
     (evaluation of affinity between base material and cells, etc.)

Imaging

• Fluorescence observation of cells using a fluorescence microscope (bioimaging)
    (cell observation using a fluorescence microscope)
• Time-lapse imaging
    (observation of cells over time)
• High-content imaging
    (screening tests using cells, such as drug and compound discovery)

Plate reader

• Measurement of fluorescence, emission, and absorption using a plate reader
     (quantification of each substance using a plate reader, etc.)

Flow cytometry

• Custom-made test of flow cytometry
     (analysis of various cells using a flow cytometer, etc.)
• Cell sorting and custom-made tests
     (separation of specific cells using a cell sorter, etc.)

Tissue dispersion/crushing

• Tissue/crushing test
    (tissue dispersion/crushing using gentleMACS, etc.)）

* The above test is an example.

* We will establish custom-made tests and experimental methods that meet the needs of our customers.
Please feel free to contact us.