CRO ACEL List of Cell-based Assays

CRO｜List of Cell-based Assays

Cosmetology field（cell-based assays）

●Melanin production suppression test
(suppression of melanin synthesis for whitening)
●Fibroblast function promotion test
(collagen promotion, hyaluronic acid production, etc.)
●Epidermal cell test: ultraviolet (UV) protection of skin
(protects skin from stress, such as UV rays)
●Skin moisturizing/barrier function gene expression analysis
(moisturizing using epidermal cells and promoting barrier function)
●Dry stress protection test for skin and cornea
(protection of dry skin and improvement of dry eye)
●An activation test of dermal papilla cells
(the functional substance that promotes hair formation)
●An activation test of various cells: a cell proliferation promotion/suppression test
(cosmetology, wound healing, suppression of cancer cells, etc.)
●Cell migration test
(wound healing, etc.)
●Atopic dermatitis improvement test
(search for substances that improve atopy, etc.)

Cosmetology field (biochemical test)

●Collagenase activity inhibition test
(control of collagen degradation)
●Tyrosinase activity inhibition test
(inhibition of melanin synthesis for whitening)

Health field(immune system)

●Immune function regulation test
(cytokine gene expression in macrophages)
●Histamine release suppression test
(excessive allergic reaction suppression by histamine)
●Cytokine production measurement test
(measurement of cytokines released from various cells)
●Multiplex measurement
(simultaneous measurement of multiple biomolecules, including cytokines)
●Immune (blood) function improvement test
(ability to promote differentiation into each blood cell)
●Enteritis suppression test
(effect of suppressing intestinal inflammation)
●Pulmonary function improvement test
(alleviation of bacterial pneumonia symptoms, etc.)

Health field (anti-obesity system)

●Fat differentiation suppression test
(search for functional substances expected to have a diet effect)
●Lipolysis promotion test
(search for functional substances expected to have a diet effect)
●Fat function improvement test
(search for functional substances expected to have a diet effect)
●Cell mitochondrial activation test
(search for functional substances that promote fat burning, etc.)
●Sugar uptake test of various cells
(search for substances with anti-diabetes and diet effects)

Health field(blood system)

●Blood vessel function improvement test
(vasodilatory action, antithrombotic action, etc.)
●Angiogenesis test
(search for wound healing agents and cancer suppressants)
●Antiarteriosclerosis test
(search for drugs that suppress the occurrence and progression of arteriosclerosis)

Health field (bone/cartilage system)

●Bone metabolism test using osteoblasts and osteoclasts
(supporting bone health)
●Chondrocyte function improvement test
(treatment and prevention of joint diseases, etc.)

Health field (liver system)

●Liver function improvement test
(correlation between drug and CYP as well as drug efficacy)

Health field (nervous system)

●Nerve cell function promotion test
(improvement effect on neurological diseases)
●NGF (Nerve growth factor) production promotion test
(nerve cell differentiation and regeneration, neurodegenerative diseases)

Health field (muscular system)

●Muscle building test using myoblasts
(increasing and maintaining muscle mass)
●Energy production promotion test using myotube cells
(energy production promotion effect of muscle mass)

Health field (others)

●Nicotinamide adenine dinucleotide phosphate oxidase activity measurement
(control of active oxygen production)

●Cancer cell infiltration inhibition test
(cancer cell expansion, metastasis suppression, etc.)

Induced pluripotent stem (iPS) cells

●Tests using
iPS cells (examination of iPS cell culture conditions and differentiation induction conditions, etc.)

Animal experiment (alternative field)

●Melanin production suppression test (three-dimensional cultured skin model)
(whitening agent, raw material for whitening cosmetics)

Gene expression analysis

●Gene expression analysis
(Ribonucleic acid [RNA] is extracted from cells and subjected to real-time polymerase chain reaction)
●Comprehensive gene expression analysis (Deoxyribonucleic acid [DNA] microarray)
(measurement of each RNA amount in cells)
●Gene knockdown test using small interfering RNA (siRNA)
(inhibition of gene expression in cells)
●Cell-forced gene expression test
(overexpression)
●Western blotting
(protein expression analysis using simple western blotting)

Base material evaluation

●Base material evaluation test
(evaluation of affinity between base material and cells, etc.)

Imaging

●Fluorescence observation of cells using a fluorescence microscope (bioimaging)
(cell observation using a fluorescence microscope)
●Time-lapse imaging
(observation of cells over time)
●High-content imaging
(screening tests using cells, such as drug and compound discovery)

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